Fluoroquinolone, Macrolide, and Ketolide Resistance in Haemophilus parainfluenzae from South Africa.
Identifieur interne : 000074 ( Main/Exploration ); précédent : 000073; suivant : 000075Fluoroquinolone, Macrolide, and Ketolide Resistance in Haemophilus parainfluenzae from South Africa.
Auteurs : Regina Esinam Abotsi [Afrique du Sud] ; Usha Govinden [Afrique du Sud] ; Krishnee Moodley [Afrique du Sud] ; Sabiha Essack [Afrique du Sud]Source :
- Microbial drug resistance (Larchmont, N.Y.) [ 1931-8448 ] ; 2017.
Abstract
Fluoroquinolones and ketolides are among the drugs of choice for the treatment of Haemophilus parainfluenzae infections. There has been a report of an emerging fluoroquinolone and telithromycin resistance in H. parainfluenzae isolates from the private sector of KwaZulu-Natal Province of South Africa that necessitates molecular investigation. The aim of this study is to characterize these resistance delineating mutations in genes commonly associated with reduced susceptibility. Ten H. parainfluenzae isolates retrieved from the sputum of 10 patients with H. parainfluenzae pneumonia were subjected to sensitivity testing by the disc diffusion and CLSI broth microdilution methods, polymerase chain reaction (PCR) and DNA sequencing of selected genes associated with resistance were carried out, while repetitive extragenic palindromic PCR (REP-PCR) was used to ascertain clonality. Fluoroquinolone resistance was attributed to the following amino acid substitutions: S84F, D88Y in GyrA, and S84Y/L, S138T, and M198 L change in ParC of the isolates. The plasmid-mediated quinolone resistance gene aac-(6')-Ib-cr was detected for the first time in four isolates of H. parainfluenzae and D420 N change was observed in ParE in one isolate. Macrolide and ketolide resistance were ascribed to the resistance genes mef (A), msr (D), and erm (B) detected in the isolates. REP-PCR analysis showed that the isolates were not clonal. All the observed resistance mechanisms are first reports in Africa. There is an emerging fluoroquinolone and macrolide resistance in H. parainfluenzae in South Africa that is attributable to known/novel resistance mechanisms, necessitating the monitoring of this pathogen as a potential opportunistic pathogen in a country with a high HIV and AIDS prevalence.
DOI: 10.1089/mdr.2016.0045
PubMed: 28103180
Affiliations:
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Durban</wicri:regionArea><wicri:noRegion>Durban</wicri:noRegion></affiliation></author><author><name sortKey="Essack, Sabiha" sort="Essack, Sabiha" uniqKey="Essack S" first="Sabiha" last="Essack">Sabiha Essack</name><affiliation wicri:level="1"><nlm:affiliation>1 Antimicrobial Research Unit, School of Health Sciences, University of KwaZulu-Natal , Durban, South Africa .</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>1 Antimicrobial Research Unit, School of Health Sciences, University of KwaZulu-Natal , Durban</wicri:regionArea><wicri:noRegion>Durban</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microbial drug resistance (Larchmont, N.Y.)</title><idno type="eISSN">1931-8448</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fluoroquinolones and ketolides are among the drugs of choice for the treatment of Haemophilus parainfluenzae infections. There has been a report of an emerging fluoroquinolone and telithromycin resistance in H. parainfluenzae isolates from the private sector of KwaZulu-Natal Province of South Africa that necessitates molecular investigation. The aim of this study is to characterize these resistance delineating mutations in genes commonly associated with reduced susceptibility. Ten H. parainfluenzae isolates retrieved from the sputum of 10 patients with H. parainfluenzae pneumonia were subjected to sensitivity testing by the disc diffusion and CLSI broth microdilution methods, polymerase chain reaction (PCR) and DNA sequencing of selected genes associated with resistance were carried out, while repetitive extragenic palindromic PCR (REP-PCR) was used to ascertain clonality. Fluoroquinolone resistance was attributed to the following amino acid substitutions: S84F, D88Y in GyrA, and S84Y/L, S138T, and M198 L change in ParC of the isolates. The plasmid-mediated quinolone resistance gene aac-(6')-Ib-cr was detected for the first time in four isolates of H. parainfluenzae and D420 N change was observed in ParE in one isolate. Macrolide and ketolide resistance were ascribed to the resistance genes mef (A), msr (D), and erm (B) detected in the isolates. REP-PCR analysis showed that the isolates were not clonal. All the observed resistance mechanisms are first reports in Africa. There is an emerging fluoroquinolone and macrolide resistance in H. parainfluenzae in South Africa that is attributable to known/novel resistance mechanisms, necessitating the monitoring of this pathogen as a potential opportunistic pathogen in a country with a high HIV and AIDS prevalence.</div></front></TEI><affiliations><list><country><li>Afrique du Sud</li></country></list><tree><country name="Afrique du Sud"><noRegion><name sortKey="Abotsi, Regina Esinam" sort="Abotsi, Regina Esinam" uniqKey="Abotsi R" first="Regina Esinam" last="Abotsi">Regina Esinam Abotsi</name></noRegion><name sortKey="Essack, Sabiha" sort="Essack, Sabiha" uniqKey="Essack S" first="Sabiha" last="Essack">Sabiha Essack</name><name sortKey="Govinden, Usha" sort="Govinden, Usha" uniqKey="Govinden U" first="Usha" last="Govinden">Usha Govinden</name><name sortKey="Moodley, Krishnee" sort="Moodley, Krishnee" uniqKey="Moodley K" first="Krishnee" last="Moodley">Krishnee Moodley</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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